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Dioxins and polychlorinated biphenyls (PCBs) are chlorinated compounds persisting in the environment and accumulating throughout the food chain, potentially causing adverse effects on humans.
Dioxins consist of two compound groups:
Certain thermal and industrial processes generate these compounds as unwanted by-products despite their lack of technological application.
Until the 1980s, many countries extensively used PCBs, which comprise 209 congeners, in industrial applications before banning them. They are categorized into dioxin-like and non-dioxin-like PCBs.
Non-dioxin-like PCBs (NDL PCBs) are persistent organic chemicals accumulating in the environment and humans, associated with various health effects. In food, the highest contamination levels occur in fish products, eggs, milk and terrestrial animal meat. In opposition, foods of plant origin show the lowest contamination levels (EFSA Study from 2010). Feed exhibits a similar trend, with higher contamination levels found in fish-derived feed and lower levels in plant or mineral-derived feed.
Dioxins and PCBs are present at low levels in many food matrices. They are resistant to biological degradation, highly lipophilic, and accumulate in numerous food products, particularly those of animal origin. Their persistence and tendency to accumulate in the food chain, notably in animal fat, continue to raise safety concerns.
Studies on the toxicity of these molecules have demonstrated that long-term exposure to these substances can lead to adverse effects on the nervous, immune, and endocrine systems. Additionally, they are considered carcinogenic, posing a significant health hazard to consumers. Fortunately, not all congeners within this wide range exhibit the same level of toxicity. The World Health Organization (WHO) has identified 17 dioxins (PCDDs and PCDFs) and 12 PCBs as highly toxic, for which a toxic equivalency factor (TEF) has been established. This factor allows the toxicity of different dioxins and dioxin-like compounds to be combined, enabling the comparison of matrices with varying amounts of congeners.
Maximum levels were established based on occurrence data compiled in the EFSA scientific report Results of the monitoring of non-dioxin-like PCBs in food and feed.
In 2018, EFSA confirmed the findings of previous assessments, stating that dietary exposure to dioxins and dioxin-like PCBs—environmental pollutants present at low levels in food and feed—is a health concern. Data from European countries indicate that EFSA’s new tolerable intake level is exceeded across all age groups.
EU Regulation 2023/915 and Directive 2002/32/EC (amended by Regulation No 277/2012) regulate the presence of dioxins and PCBs in food and feed, setting maximum levels for individual residues and their sums.
The maximum levels apply to 17 PCDD/PCDFs, 12 dioxin-like PCBs, and 6 non-dioxin-like PCB indicators, collectively representing more than half of those typically found in food and feed.
Mérieux NutriSciences provides comprehensive and reliable analytical services fully compliant with current requirements on dioxins and PCBs in food, feed, and environmental matrices.
Our dedicated team offers analytical packages applying official EPA methods via HRGC-HRMS, with results expressed in toxicity factors and TEQ units, calculated using appropriate analytical-toxicological conversion factors.

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