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PFAS (per- and polyfluoroalkyl substances) are a family of thousands of synthetic chemicals. Industries have been producing these since the 1940s. The most well-known compounds include PFOS, PFOA, and their salts. These substances have unique properties, such as resistance to water, oils, fats, and high temperatures, which led to their widespread use. However, these same properties have raised significant environmental and human health concerns.
PFAS are persistent contaminants. Scientists call them “Forever Chemicals” or “Persistent Mobile Organic Compounds” because the fluorine-carbon bond in these substances is extremely difficult to break down in both humans and the environment.
PFAS pose risks to human health and the environment. They are present in various matrices, including water, soil, air, waste, food, and food contact materials.

Authorities have added new PFAS molecules to the list of POPs, and legislation related to food and packaging is evolving. Due to the mobility of these contaminants across various matrices, authorities will likely introduce more regulations on PFAS concentration limits and shared analytical methods in the coming years.

For water intended for human consumption, EU Directive 2020/2184 addresses emerging contaminants. These are substances that are minimally or not at all regulated in a harmonized way in Europe, but pose serious safety concerns due to their widespread presence and associated health risks.
Using various international and accredited methods, we perform PFAS analysis in drinking water, as well as in wastewater and groundwater.

The POPs list includes PFOS, PFOA, PFHxS, along with their salts and derivatives. This applies to the classification and management of waste, including its disposal in landfills, and regulates waste that consists of, contains, or is contaminated by these substances.
We monitor PFAS in waste in accordance with regulations, particularly focusing on those PFAS considered as POPs.

PFAS can spread through environmental emissions. Monitoring their presence in the air is essential to understanding their mobility across different environmental compartments.
We use the US EPA OTM 45 method, which is the primary reference for sampling PFAS in air emissions. We use this performance-based method to collect and analyze specific semi-volatile and particulate PFAS from stationary emission sources.

Studies show that PFAS concentrations in soils at contaminated sites are often significantly higher than in groundwater. PFAS have been detected in soils worldwide, indicating that soil acts as a major reservoir.
This is a critical issue that we can monitor with specialized analytical methods.

Firefighting foams, which were—and in some cases still are—made with PFAS, are responsible for many instances of PFAS contamination in Europe and the US, affecting both soil and drinking water. As a result, regulatory initiatives are evolving globally to limit their use in such applications.
We analyze firefighting foams, along with water and soil from areas where they were used. Our approach ranges from targeted techniques to broader methods, including precursor analysis and projects aimed at supporting management plans to address identified risks.

Mérieux NutriSciences has extensive knowledge of PFAS, thanks to years of experience in areas contaminated by these substances. We participate in technical panels and proficiency tests, and we regularly collaborate with stakeholders and working groups to discuss PFAS techniques, limits, and monitored molecules.
For this reason, we conduct analyses in various matrices beyond environmental samples, such as:
The most well-known PFAS compounds are monitored using conventional targeted analysis. This approach provides quantitative data and ensures compliance with legislation when a precise list of molecules is available.
The new challenge is managing a wide range of emerging contaminants, including dangerous PFAS or their precursors, while ensuring they remain part of the target.
Mérieux NutriSciences can perform targeted analysis on 100 PFAS using LC/MS/MS, LC-HRMS, and GC/MS/MS.
This method is useful as an initial screening to provide a quick overview of the amount of fluorinated organic compounds present. It can be followed by targeted analysis of individual PFAS. The analysis is performed in aqueous matrices using combustion ion chromatography (CIC).
The method is recommended by the EPA under the Clean Water Act (CWA), and since July 2023, AOF has been mandatory in wastewater for many industries in France.
The total fluorine in an entire sample is measured through integrated direct combustion. In combination with a separate measurement of inorganic fluorine extracted in water before combustion, it is possible to determine total, organic, and inorganic fluorine levels.
This is a rapid, accurate, and highly sensitive method, although not specific. It serves as a reference to demonstrate the absence of intentionally added fluorine, such as in packaging or firefighting foams.
This method monitors many PFAS precursors and intermediates. These can be converted into PFAS-related chemicals through oxidation. The TOP assay converts them into stable PFAS with established analytical standards.
To study the vast quantity of PFAS compounds that might be present in a sample, the analysis can be limited to a screening list of potential candidates, even in the absence of standards.
Among the thousands of molecules identified, only those matching the list of interest or “suspected” compounds will be evaluated and confirmed at varying levels. Suspect screening focuses on the so-called “known unknowns,” where names and structures are well-defined and suspected to be present in the sample.
The next step is non-target analysis. The challenge for the coming years will be preparing to investigate the “unknown unknowns” (compounds that cannot be predicted) through high-level instrumental analysis. This will allow for possible hypotheses about the molecules present, based on the obtained results.

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